D360-3
Anti-Mincle (Human) mAb (Functional Grade) Anti-Mincle (Functional Grade)——北京博迈斯科技发展有限公司
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M047-10
Anti-Myc-tag mAb-Magnetic Agarose Anti-Myc-tag-Magnetic Agarose——北京博迈斯科技发展有限公司
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M090-5
Rat IgG2b (isotype control)-PE Rat IgG2b (isotype control)-PE——北京博迈斯科技发展有限公司
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M132-10
Anti-HA-tag mAb-Magnetic Agarose Anti-HA-tag-Magnetic Agarose——北京博迈斯科技发展有限公司
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M165-11
Anti-RFP mAb-Magnetic Beads Anti-RFP-Magnetic Beads——北京博迈斯科技发展有限公司
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M167-10
Anti-V5-tag mAb-Magnetic Agarose Anti-V5-tag-Magnetic Agarose——北京博迈斯科技发展有限公司
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M168-3M
Anti-monomeric Kusabira-Orange 2 mAb Anti-monomeric Kusabira-Orange 2——北京博迈斯科技发展有限公司
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M182-3M
Anti-Keima-Red mAb Anti-Keima-Red——北京博迈斯科技发展有限公司
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K7116-100
K7116-100? Angiopoietin-2 Microplate (Item A) coated with anti-human Angiopoietin-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Angiopoietin-2) ? Assay Diluent (Item E) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent (5X) ? Detection Antibody Angiopoietin-2 (Item F), biotinylated anti-human Angiopoietin-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineAngiopoietin-2 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human ANGPT2 in serum, plasma, urine & cell culture supernatants. Detection Range: 10 pg/ml - 3000 pg/ml. 100 assays. BioVision’s Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2. This assay employs an antibody specific for human Angiopoietin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, Angiopoietin-1, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13,IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Angiopoietin-2 is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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6789-100
NGFR Antibody (Clone # 8G3G10) Host:Mouse Antibody Type:Monoclonal Species Reactivity:Human. Application:Western blot, IF, FACS. WB: 1:500 – 1:2000, IHC: 1:50 – 1:100, IF: 1:100.
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BE0282-50MG
InVivoMab anti-mouse IL-3 bioxcell InVivoMab-北京博迈斯科技发展有限公司
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K4755-100
K4755-100? Serum & plasma ? Cell culture supernatants ? Urine Angiostatin (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human Angiostatin in Serum, Plasma, urine & Cell culture supernatants. Detection Limit: less than 20 ng/ml. 100 assays.BioVision’s Human Angiostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiostatin. This assay employs an antibody specific for human angiostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and angiostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human angiostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of angiostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of angiostatin is typically less than 20 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4914-100
K4914-100? 1 plate coated with human ANGPTL3 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial human ANGPTL3 Standard (lyophilized) ? 1 vial human ANGPTL3 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL3 (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ANGPTL3 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.156–10 ng/ml and Detection limit 75 pg/ml. 100 Assays. The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460-amino acid and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of human ANGPTL3 in biological fluids. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.
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K4915-100
K4915-100? 1 plate coated with mouse ANGPTL3 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial mouse ANGPTL3 Standard (lyophilized) ? 1 vial mouse ANGPTL3 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film) ? 1 plate coated with mouse ANGPTL3 AntibodyCell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL3 (mouse/rat) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of mouse/ rat ANGPTL3 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.016 – 1 ng/ml and Detection limit 15 pg/ml. 100 Assays. The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460-amino acid and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of mouse/ rat ANGPTL3 in biological fluids. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples. The assay range is 0.016 – 1 ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 15 pg/ml.
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K4916-100
K4916-100? 1 plate coated with human ANGPTL6 Antibody ? Wash Buffer 10X ? Diluent 5X ? Detection Antibody ? Detector 100X (HRP Conjugated anti-rabbit IgG) ? human ANGPTL6 Standard (lyophilized) ? human ANGPTL6 QC sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL6 (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ANGPTL6 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 1.56 – 100 ng/ml and Detection limit 1.2 ng/ml. 100 Assays. Seven angiopoietin-like proteins (ANGPTLs) share the characteristic protein structure of the angiopoietin family (ANG), but differ in their inability to bind antiopoietin receptor, Tie-2. ANGPTL6 was originally named angiopoietin-related growth factor (AGF) having an N-terminal coiled-coil-like domain and a C-terminal fibrinogen-like domain, both of which are conserved in ANG. It is a circulating protein secreted by liver that induces angiogenesis by direct effect of epidermal ANGPTL6 on endothelial cells and proliferation of skin cells, and thereby promotes wound healing. 80% of Angptl6 -/- mice died at about embryonic day 13. The surviving null mice developed marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure relative to controls. Conversely, mice with constitutive overexpression of ANGPTL6 showed leanness and increased insulin sensitivity resulting from increased energy expenditure, and were also protected from high-fat diet-induced obesity, insulin resistance, and non-adipose tissue steatosis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL6 in biological fluids. A monoclonal antibody specific for ANGPTL6 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL6 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL6 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL6 in the samples. The assay range is 1.56 – 100 ng/ml ANGPTL6/ml. The lowest level of ANGPTL6 that can be detected by this assay is 1.2 ng/ml.
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