K4696-100
K4696-100? Plate Coated with ApoE Ab ? Assay Diluent ? Wash Buffer A (10x) ? Wash Buffer B (10x) ? rh ApoE Standard ? Detection Antibody (100x) ? HRP Conjugate (100x) ? TMB Substrate ? Stop Solution ? Plate Sealer ? Serum and plasma ? Cell lysate and cell culture supernatant ? Cerebrospinal Fluid (CSF) Apolipoprotein E (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of human ApoE in serum, plasma, cell lysate & cell culture supernatant, & cerebrospinal fluid. Detection range: 25 ng to 1.6 μg/ml. 100 assays. BioVision’s Human Apolipoprotein E (ApoE) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of human ApoE. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. It is synthesized mainly in liver, while it has also been identified in the brain, kidneys, and spleen. In the nervous system, ApoE is synthesized in non-neuronal cell types, most notably astroglia and microglia, while neurons preferentially express its receptors. Defects in ApoE lead to familial dysbetalipoproteinemia (increased plasma cholesterol and triglycerides). More recently, ApoE has been implicated in several biological processes not directly related to lipoprotein transport, including Alzheimer's disease, immunoregulation, and cognition. The assay employs an antibody specific for human ApoE coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms. Sensitivity of the kit is 25 ng/ml and detection range is from 25 ng to 1.6 μg/ml. Recovery inside this range is between 86 and 111% (average recovery is 102%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
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K4699-100
K4699-100? Serum and plasma ? Cell lysate and cell culture supernatant ? Cerebrospinal Fluid (CSF) Apolipoprotein E4/Pan-ApoE (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of human ApoE4 in serum, plasma, cell lysate & cell culture supernatant, & cerebrospinal fluid. Detection range: 50 ng to 800 ng/ml. 100 assays. BioVision’s Human Apolipoprotein E4 (ApoE4) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for quantitative measurement of human ApoE4. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. ApoE exists as three major isoforms, including ApoE2, ApoE3, and ApoE4. Recently, ApoE4 has been implicated in Alzheimer's disease (AD). ApoE4 is the first risk gene identified in Alzheimer's research, and remains the gene with strongest impact. Everyone inherits a copy of ApoE gene from each parent. Those who inherit one copy of ApoE4 have an increased risk of developing AD (about a quarter of the human population). Those who inherit two copies of ApoE4 have an even higher risk (about 2% of humans with up to 10 times higher risk). In addition to raising risk, ApoE4 may tend to make AD symptoms appear at a younger age. The assay employs an antibody specific for human ApoE4 coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE4 present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE4 specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE4. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 25 ng/ml and detection range is from 50 ng to 800 ng/ml. Recovery inside this range is between 83 and 104% (average recovery is 93%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
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K3599-100
K3599-100? Aromatase Ab-coated plate ? Human Aromatase standard, lyophilized ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from human tissues or cells ? Serum, plasma (EDTA, Heparin) Aromatase (Cytochrome P450 19A1) human ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Aromatase in tissues, cells, plasma, serum or microsomes. Sensitivity: 0.156 ng/ml. Detection Range: 0.156 ng/ml – 10 ng/ml. 100 assays. Aromatase (Cytochrome P450 19A1 or CYP19A1) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in steroidogenic tissue (gonads, breast, adipose tissue, brain, placenta etc.). Aromatase is responsible for conversion of androgens into estrogens and is important in normal sexual development as well as breast, endometrial and other cancers. BioVision’s human Aromatase ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A human Aromatase-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to Aromatase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the human Aromatase captured onto the plate. The concentration of Aromatase in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.156 ng/ml – 10 ng/ml. Sensitivity: 0.156 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Recovery: Serum (~90%), plasma (EDTA = ~90%, Heparin = ~95%).
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K3560-100
K3560-100? Aromatase Ab-coated plate ? Rat Aromatase standard, lyophilized ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from rat tissues or cells ? Serum, plasma (EDTA, Heparin) Aromatase (Cytochrome P450 19A1) rat ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of rat Aromatase in tissues, cells, plasma, serum or microsomes. Sensitivity: 1.56 ng/ml. Detection Range: 1.56 ng/ml – 100 ng/ml. 100 assays. Aromatase (Cytochrome P450 19A1 or CYP19A1) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in steroidogenic tissue (gonads, breast, adipose tissue, brain, placenta etc.). Aromatase is responsible for conversion of androgens into estrogens and is important in normal sexual development as well as breast, endometrial and other cancers. BioVision’s rat Aromatase ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat Aromatase-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to Aromatase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the rat Aromatase captured onto the plate. The concentration of Aromatase in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 1.56 ng/ml – 100 ng/ml. Sensitivity: 1. 56 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Recovery: Serum (~90%), plasma (EDTA = ~90%, Heparin = ~85%).
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K4744-100
K4744-100? BMP-2 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-2. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-2. ? Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-2 (Item F): 2 vial of biotinylated anti human BMP-2 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 200x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-2 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-2 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 45 pg/ml. 100 assays.BioVision’s Human BMP-2 (Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-2. This assay employs an antibody specific for human BMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-2 is typically less than 45 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. In this ELISA formate, the pair antibody shows less than 1% cross-reactivity with rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 or rhBMP-7.
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K4745-100
K4745-100? BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-4. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 80x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-4 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-4 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 15 pg/ml. 100 assays.BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4746-100
K4746-100? BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-4. ? Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent. ? Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Detection Antibody (Item F) & HRP-Streptavidin Concentrate (Item G) diluent. ? Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 80x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Cell lysate buffer (Item J): 5 ml 2X cell lysate buffer. ? Cell lysate & tissue lysateBMP-4 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-4 in cell & tissue lysates. Detection Limit: less than 15 pg/ml. 100 assays.BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4747-100
K4747-100? BMP-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-5. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-5. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-5 (Item F): 2 vial of biotinylated anti human BMP-5 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 300x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma (BMP-5 concentration in human normal serum/plasma is pretty low, it may not be detected in this assay) ? Cell culture supernatants ? Urine BMP-5 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-5 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 0.25 ng/ml. 100 assays.BioVision’s Human BMP-5 (Bone morphogenetic proteins-5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-5. This assay employs an antibody specific for human BMP-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-5 is typically less than 0.25 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4748-100
K4748-100? BMP-6 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-6. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-6. ? Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasma/cell culture medium/urine) & reagent diluent. ? Detection Antibody BMP-6 (Item F): 2 vial of biotinylated anti-human BMP-6 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 1,500x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-6 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-6 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 150 pg/ml. 100 assays.BioVision’s Human BMP-6 (Bone morphogenetic proteins-6) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-6. This assay employs an antibody specific for human BMP-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-6 is typically less than 150 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4749-100
K4749-100? BMP-7 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-7. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-7. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-7 (Item F): 2 vial of biotinylated anti human BMP-7 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 400x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-7 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-7 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 10 pg/ml. 100 assays.BioVision’s Human BMP-7 (Bone morphogenetic proteins-7) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-7. This assay employs an antibody specific for human BMP-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-7 is typically less than 10 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7427-100
K7427-100? Plate coated with CA19-9 MAb ? CA19-9 Standard ? CA19-9 Assay Buffer ? Enzyme Conjugate Conc. (12x) ? CA19-9 Conjugate Diluent ? Wash Concentrate (20X) ? TMB Substrate ? Stop Solution SerumCA19-9 (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Cancer Antigen 19-9 (CA19-9) in serum. 100 assays. CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recently reports indicates that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA19-9 is suggestive of gallbladder neoplasm in the setting of inflammatory gallbladder disease. It has been shown that a persistent elevation in serumCA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment. The CA19-9 ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA19-9 molecule for immobilization (on the microtiter wells). Another CA 19-9 monoclonal antibody conjugated to horseradish peroxidase (HRP) is in the antibody-enzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA19-9 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate incubation steps at 37°C for 90 min., the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 min., resulting in development of blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA19-9 is directly proportional to the color intensity of the test sample.
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K4803-100
K4803-100? CA-125 Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CA-125 Standard (Item C) ? Assay Diluent (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (800x ) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCancer Antigen 125 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA-125 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.6 U/ml - 400 U/ml. 100 assays. BioVision’s Human Cancer Antigen 125 (CA-125) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-125. This assay employs an antibody specific for human CA-125 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA-125 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA-125 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA-125 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA-125 is typically less than 0.6 U/ml. Detection Range: 0.6 U/ml – 400 U/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4804-100
K4804-100? CA15-3 Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CA15-3 Standard (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (200x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCancer Antigen 15-3 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA15-3 in serum, plasma, urine & cell culture supernatants. Detection Range: 4 mU/ml - 1000 mU/ml. 100 assays. BioVision’s Human Cancer antigen 15-3 (CA15-3) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA15-3. This assay employs an antibody specific for human CA15-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA15-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA15-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA15-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA15-3 is typically less than 4 mU/ml. Detection Range: 4 mU/ml – 1000 mU/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4805-100
K4805-100? CEA Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CEA Standard (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (1000x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCarcinoembryonic Antigen (CEA) (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CEA in serum, plasma, urine & cell culture supernatants. Detection Range: 0.2 ng/ml - 250g/ml. 100 assays. BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CEA is typically less than 200 pg/ml. Detection Range: 0.2 ng/ml – 250 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K3072-100
K3072-100? CD40 Microplate (Item A) coated with anti-human CD40, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD40) ? Assay Diluent D (Item K) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent. 5x concentrated ? Assay Diluent B (Item E) for detection antibody & HRP-Streptavidin diluent. 5x concentrated ? Detection Antibody CD40 (Item F), biotinylated anti-human CD40 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineCD40 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD40 in serum, plasma, urine & cell culture supernatants. Detection Range: 50 pg/ml - 40000 pg/ml. 100 assays. BioVision’s Human CD40 (TNFRSF5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CD40 is typically less than 50 pg/ml. Detection Range: 50 pg/ml – 40000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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